2022年7月2日 11:00～12:00 沖縄コンベンションセンター 展示棟 ポスター会場1
3P-257
ASD血漿中のTNF-α発現とASD諸症状との相関解析
Correlation analysis of TNF-α expression in plasma of patients with ASD and the symptoms of ASD.
*山内 崇平(1)、萱嶋 善徳(1)、今井 一彰(1)、神川 浩平(1)、高田 涼平(1)、小森 崇史(1)、石田 理緒(1)、岸本 直子(1)、鳥塚 通弘(1)、牧之段 学(1)
1. 奈良県立医科大学
*Takahira Yamauchi(1), Yoshinori Kayashima(1), Kazuaki Imai(1), Kohei Kamikawa(1), Ryohei Takada(1), Takashi Komori(1), Rio Ishida(1), Naoko Kishimoto(1), Michihiro Toritsuka(1), Manabu Makinodan(1)
1. Nara medical university
Keyword: Autism spectrum disorder, tumor necrosis factor-α
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impaired social interaction, poor communication skills, and repetitive or restrictive patterns of behavior and interests. The diagnosis of ASD typically relies on a clinical interview using the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), and the most accurate diagnosis is achieved using non-biological tools, namely the Autism Diagnostic Observation Schedule-2 (ADOS-2) and Autism Diagnostic Interview-Revised (ADI-R). Although these tools are highly powerful, for more objective assessments, biological diagnostic tools are also necessary. The etiology of autism spectrum disorder (ASD) is complex, and its pathobiology is characterized by enhanced inflammatory activities; however, the precise pathobiology and underlying causes of ASD remain unclear. We previously reported that tumor necrosis factor-α (TNF-α) expression ratio of M1/M2 macrophages has the potential to increase the true-positive ASD diagnosis rate with high sensitivity. However, there is the problem that it takes a lot of time and effort to make macrophages from blood samples, and we investigated the relationship between the expression level of TNF-α and ASD diagnosis using plasma samples. TNF-α Expression in plasma was not significantly different between patients with ASD and typical developing individuals. The score of social affect on the ADOS-2, indicating social interaction impairment, was negatively correlated with TNF-α expression level in peripheral blood plasma, but the score of restricted and repetitive behaviors was not correlated with the level of TNF-α. | | 2022年7月2日 11:00～12:00 沖縄コンベンションセンター 展示棟 ポスター会場1
3P-258
マウス脳スライス標本における低張液暴露時のTransient receptor potentialの発達過程を脳細胞浮腫とカルシウム上昇から探る
Development of transient receptor potential in mouse brain slice preparations during exposure to hypotonic solution as revealed by brain cell edema and calcium elevation
*高橋 奈々恵(1)
1. 東京医科大学八王子医療センター
*Nanae Takaahshi Takahashi(1)
1. Tokyo medical University Hachioji medical center, Tokyo, Japan
Keyword: Transient receptor potential (TRP) channels, developmental process
【Aim】Transient receptor potential (TRP) channels are a large family of ion channel receptors in the plasma membrane, consisting of six subfamilies and 27 channels in humans. There are no studies on the developmental process of TRP channels and their changes with aging. In addition, we have not found any studies that classify the process of brain development in mice based on the extensibility of the cell membrane. In the present study, we aimed to examine the difference of channel response between age groups in TRP channels activated by hypotonic solution. 【Method】Ca 2+concentrations ([Ca 2+]i) in the lateral cerebral cortex (LCC) and hippocampal CA1 (CA1) regions were measured in mouse brain slices, and brain edema was also measured. Brain slices were loaded with the fluorescence Ca2+ indicator fura-2, and cell volume and [Ca2+ ]i in the lateral cerebral cortex (LCC) and hippocampal CA1 (CA1) regions were measured simultaneously. hippocampal CA1(CA1) regions were measured simultaneously during exposure to hypotonic stress using Ca2+ insensitive (F360) and Ca 2+sensitive Ca2+ elevation was almost absent in infants, while Ca2+ elevation developed rapidly in young adults2+. 【Result】As for the brain cell edema, it was confirmed that the edema also became less likely to occur with significantly larger age. The age-dependent responses of TRP channels distributed on the cell membrane were significantly different. 【Conclution】The difference in the development of cell membranes at different ages may be one of the reasons for the present results. We would like to discuss this further in the future. |
