大会企画 教育セミナー
Educational Seminar |
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| 2020/9/10 15:10～16:00 Zoom A
ES
マウスとフェレットを用いた大脳皮質の形成と進化のメカニズム解析
Molecular investigation of the development and evolution of the cerebral cortex using mice and ferrets
*河崎 洋志1
1. 金沢大学医学系
*Hiroshi Kawasaki1
1. Graduate School of Medicine, Kanazawa University
Expansion and folding are prominent features of the mammalian cerebral cortex during evolution. To investigate the mechanisms underlying the development and evolution of the mammalian cerebral cortex, we developed a genetic manipulation technique for the cerebral cortex of gyrencephalic carnivore ferrets using in utero electroporation. Genes-of-interest can be expressed in the ferret cortex rapidly and efficiently. We also demonstrated that genes-of-interest can be knocked out in the ferret cortex by combining in utero electroporation and the CRISPR/Cas9 system. Using our technique, we previously reported that FGF signaling is crucial for cortical folding and proliferation of neural progenitors. Here we show the role of sonic hedgehog (Shh) signaling in the development of the ferret cerebral cortex. We found that activation of Shh signaling resulted in additional folding in the ferret cerebral cortex. Consistently, suppression of Shh signaling by expressing Hhip-DeltaC22 inhibited cortical folding. Interestingly, we found that Shh signaling was more activated in the developing cerebral cortex of ferrets than that of mice, suggesting that Shh signaling is involved in the evolution of the cerebral cortex. In addition to cortical folding, we would like to introduce our recent findings about the evolution of cortical fibers by comparing mice and ferrets. Our technique for the ferret cerebral cortex should be useful for investigating the mechanisms underlying the development and evolution of the cerebral cortex.
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