2022年6月30日 17:10～17:35 沖縄コンベンションセンター 劇場棟 第1会場
JSN-AL1
The mechanisms of stress-induced anxiety responses revealed by brain-wide neuronal activation mapping
*笠井 淳司(1)
1. 大阪大学大学院薬学研究科
*Atsushi Kasai(1)
1. Grad Sch Pharm Sci, Osaka Univ, Osaka, Japan
Keyword: stress, claustrum, anxiety, depression
The processing of stress responses including anxiety involves brain-wide communication among cortical and subcortical regions; however, the mechanisms underlying stress induced anxiety responses remain elusive. To address this issue, we have developed a high-speed imaging apparatus based on automated mechanical sectioning and confocal imaging, named FAST (block-FAce Serial microscopy Tomography). FAST acquires submicrometer-resolution images of whole mouse brain in a few hours. Then, using FAST, we conducted brain-wide activation mapping in an immediate early gene reporter mouse, Arc-dVenus mouse, after exposure to a stressor. A combined approach using brain activation mapping and machine learning showed that the claustrum (CLA) activation serves as a reliable marker of exposure to acute stressors. Moreover, in the Fos2A-iCreERT2 knock-in mice, also known as TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic activation of the CLA neuronal ensemble tagged by acute social defeat stress (DS) elicited anxiety-related behaviors, whereas silencing of the CLA ensemble attenuated DS-induced anxiety-related behaviors. In addition, silencing of the CLA ensemble during repeated DS attenuated depression-like behaviors. These results indicate that a claustrum ensemble bidirectionally controls stress-induced anxiety-related behaviors, and its inactivation can serve as a preventative strategy to increase stress resilience. | | 2022年6月30日 17:40～18:05 沖縄コンベンションセンター 劇場棟 第1会場
JSN-AL2
Neural circuit mechanisms for episodic memory formation in the entorhinal cortical-hippocampal networks
*北村 貴司(1)
*Takashi Kitamura(1)
1. Department of Psychiatry and Neuroscience, University of Texas Southwestern Medical Center
Keyword: Neural Circuit, Episodic Memory Formation, EC-HPC Network
In humans and animals, episodic memory requires the concerted association of objects, space and time coordinated by the entorhinal cortex (EC)-hippocampal (HPC) network. It remains unknown how the EC-HPC network spatially and temporally associates the diverse set of information (where, what, when and who) in a single episode. Using advanced mouse genetics combined with viral tracing, in vitro and in vivo electrophysiology, in vivo calcium imaging and optogenetics, I tried to identified specific neuronal circuits for encoding each component (where, what, when and who) of episodic memory. In my talk, I present neural circuits mechanisms for remembering time and space for the memory formation in the EC-HPC networks. |
